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Stabilization Test of Deodorant(DEOMASTER-DCX)
◈ Test Institute : Test Institute Appointed by the Ministry of
Welfare Department, Japan
- Address : 1429-2-B Dong-Gi-Yon-Je Tokyo Food Technology Institute
Under the incorporated body of Tokyo Food & Hygiene Association
① Mouse Test
- Acute Intraperitoneal Toxicity Test :
After mouse intraperitoneal 20 ml/kg input, lethal case was not
found.
- Skin Stimulation Test :
When applying the product to mouse skin, there was no abnormality
in the skin.
- Ocular Mucous Membrane Stimulation Test :
When dripping on the mouse eye, there was no abnormality in the
ocular membrane.
② Analysis Test
- Arsenic : not detected (as AS2O3, below 0.5 ppm)
- Heavy Metal : not detected (as Pb, below 20 ppm)
- Cyan : not detected
- Methanol : not detected
③ AMES test (Carcinogenicity test)
- Mutagenicity of the specimen is not recognized within the range
of this test.
(Test Method : according to The Announcement No. 77 of The Department
of Labor, Japan)
④ Acute Oral Test(The Foundation, The Japan Food Analysis Centre)
1) Test Result
Table 1. Death and Death Ratio
|
Sex
|
Test group
(NO.)
|
Dosage
(mg/kg)
|
Mortality by time
|
Mortality (%)
|
LD50 (mg/kg)
|
|
Hour
|
Day
|
|
5
|
15
|
1
|
2
|
3
|
4
|
5
|
6
|
14
|
|
male
|
1
|
1,160
|
0/5
|
0/5
|
0/5
|
0/5
|
0/5
|
0/5
|
0/5
|
0/5
|
0/5
|
0
|
>2,000
|
|
2
|
1,380
|
0/5
|
0/5
|
0/5
|
0/5
|
0/5
|
0/5
|
0/5
|
0/5
|
0/5
|
0
|
|
3
|
1,660
|
0/5
|
0/5
|
0/5
|
0/5
|
0/5
|
0/5
|
0/5
|
0/5
|
0/5
|
0
|
|
4
|
2,000
|
0/5
|
0/5
|
0/5
|
0/5
|
0/5
|
0/5
|
0/5
|
0/5
|
0/5
|
0
|
|
female
|
1
|
1,160
|
0/5
|
0/5
|
0/5
|
0/5
|
0/5
|
0/5
|
0/5
|
0/5
|
0/5
|
0
|
>2,000
|
|
2
|
1,380
|
0/5
|
0/5
|
0/5
|
0/5
|
0/5
|
0/5
|
0/5
|
0/5
|
0/5
|
0
|
|
3
|
1,660
|
0/5
|
0/5
|
0/5
|
0/5
|
0/5
|
0/5
|
0/5
|
0/5
|
0/5
|
0
|
|
4
|
2,000
|
0/5
|
0/5
|
0/5
|
0/5
|
0/5
|
0/5
|
0/5
|
0/5
|
0/5
|
0
|
2) Intoxication Symptom
Intoxication symptom or death was not found in both of male and
female mice after the treatment. Feedstuff was supplied to test
mice 3 hours after the treatment but all mice started intake.
3) Autopsy Findings
After the termination of the test, all mice were slaughtered to
perform autopsy with naked eyes, but there was no abnormality
in important organs in both of male and female mice.
4) Considerations
According to the Acute Toxicity Test Method in OECD Guideline
(April 11, 1986), maximum dosage of the test product to the mouse
is set to 2,000 mg/kg.
In this test, 5.8, 6.9, 8.3 and 10% (w/v) solutions are prepared
to prescribe 20 ml/kg of the solution to mice. Upper limit of
the test product is set to 2,000 mg/kg. Test ratio was 1.2 and
the test was performed in 4 levels to both of male and female
mice.
From the result, noticeable intoxication symptom was not found
in any dosage. There was no lethal case. In addition, changes
in the weight of mice during the test period were studied, but
both of male and female mice did not represent particular trend
in the relation with dosage of test product and there was no significant
difference in the growth and development.
From the above results, we found that oral LD50 of the test
product was over 2,000 mg/kg
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