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KERASOFTⓡ ?
◈ The keratinolytic protease produced from microorganism makes
skin soft and increases whitening effect by smoothly maintaining
the metabolism of skin through removal of the keratin which is
most of aged skin surface.
◈ The added keratinolytic protease has the various abilities such
as
maintenance of soft skin by removal of horny layer and impurities
of protein,
degradation of aged tissue, stimulation of skin metabolism, and
absorption of
nutrition etc.
◈ Advantages of keratinase for a application of cosmetics
- The keratinase makes skin color light up through stimulating
turnover by
degradation of keratin, desmosome that is the main component of
epidermis.
- It is very low sensitive by using an enzymatic peeling but not
the chemical
peeling.
- Effect of Anti-acne by opening the pores of skin surface
Specifications
◈ Appearance : Pale yellow, Granule powder
◈ Composition : Concentrate of Bacillus sp. culture broth (Powder)
◈ Quality standard
- Keratinase activity(powder) about 3,500 PU/g
- Bacteria count below 10³ CFU/g
- E.coli negative
- Heavy metal(Pb) below 10 ppm
- Arsenic(As) below 2 ppm
◈ Keratinase Activity
The keratinase activity is measured by determining the quantity
of tyrosine hydrolyzed after reaction with enzyme with casein
as a substrate in absorbance (660nm).
◈ Product’s quality
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Analytical Tests
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Specifications
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Results
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| Name |
Nattokinase |
- |
| Origin/Nature Description |
Bacillus. natto |
- |
| Active Ingredient |
18,000 FU/g |
>18,000 FU/g |
| Odor |
Faint characteristic odor |
Passed |
| Appearance |
Yellowish brown powder |
Passed |
| Heavy Metal (Pb) |
≤ 10 ppm |
< 0.03 ppm |
| Aresnic (As) |
≤2 ppm |
< 0.1 ppm |
| pH(1∼10, 20 ℃ ) |
6.0∼8.0 |
7.3 |
| Moisture Content |
< 5 % |
Passed |
| Microbes |
≤ 10³ CFU/g |
80 CFU/g |
| E-coli. |
Negative |
Passed |
Uses & Functions
◈ Additives of a functional cosmetics
◈ Removal of a horny layer of skin by substrate specificity
◈ Removal of keratin and impurities of protein
◈ Increase of whitening effect
◈ Nontoxic, High activity
◈ Stimulation of skin metabolism
◈ Maintenance of soft skin
Uses & Functions
◈ Store/Preservation period
- Liquid : refrigeration for one year (quickly use after thawing)
- Concentrate : dark refrigeration for one year
- Powder : dark refrigeration for one year
◈ Packing Unit
- 1Kg/1 pack(Vacuum packing)
- 5Kg/1 Box
Test Results
Casein and Skim milk agar plate assay
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|
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0.06% casein
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2.0 % Skim milk
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SDS-PAGE of keratin degradation pattern
(A) Reaction with protease (B) Reaction
with papain
Lane 1 : size marker
2 : keratin
3 : After 10 min
4 : After 20 min
5 : After 30 min
- Changes of skin coloration index
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|
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1 : Gel base
2 : 2% protease
3 : 5% protease
4 : 2% papain
5 : 2% AHA
6 : Control |
- Skin replicas before and after continual use of cleansing preparation
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(A) Before
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(B) After 14 days
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